![]() ![]() Test on a dot blot at several concentrations.Validate target and antibody combinations using checkerboard screening protocolsīio-Rad now offers PrecisionAb™ Validated Western Blotting Antibodies for superior performance in western blot detection.Īntibody activity loss due to long-term or improper storage.Check datasheet for recommended conditions.Bio-Rad now offers high-quality antibodies for all applicationsĪntibody not suitable for western blotting Test and optimize antibody on dot blots.Lower temperature, reduce detergent concentration, reduce ionic strength.Increase the antibody concentration 2–4 times higher than initial trial. ![]() Wrong concentration of antibody or low affinity to the target protein Repeat experiment with the correct antibody combination. ![]()
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